자산 1
ENG KOR
ENG KOR
  • PCR > cDNA Synthesis

    WizScript™ RT FDmix

    WizScript™ RT FDmix (dT20 or Hexamer) is a complete system for the efficient synthesis of the first-strand cDNA from RNA templates and combines all the reagents necessary for successful cDNA synthesis in a  convenient individually aliquot and lyophilized in single-tube, one-step format. The FDmix formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps.
    WizScript™ RT FDmix included MMLV RTase (RNase H-) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade the template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. An enhanced buffer allows for RT reaction temperatures up to 50°C.

    • Overview
      • Ready-to-use dried type
      • Easy reaction setup (pre-aliquot in 8-strip tube)
      • Minimal optimization requirements
      • Room-temperature reaction set-up
      • Applied unique enhancing & stabilizing technology
      • Eliminating extra pipetting steps and reducing contamination risk from
      • multiple reagents and providing better reproducibility
    • Ordering Information
      Cat No. Size Remark
      W2204 96 rxn Includes oligo dT20 primer
      W2205 96 rxn Includes random hexamer
    • Technical Data
    • Applications
      • First Strand cDNA synthesis for RT-PCR
      • Construction of cDNA libraries
      • Generation of probes for hybridization
      • Antisense RNA synthesis
    • Storage & Shipping
      Shipped at room temperature
      Store at -20ºC, upon receipt