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  • PCR > Master Mix (Solution type)

    WizPure™ Pfu 2X Master

    WizPure™ Pfu 2X Master is a ready-to-use Pfu DNA polymerase pre-mixes are the innovation for convenience of your routine PCR as you just have to add your template DNA besides your primers & put the tubes for amplification. WizPure™ Pfu 2X Master is an optimized, ready-to-use PCR mixture of Pfu DNA Polymerase, PCR buffer, MgCl2, and dNTPs. This Pfu 2X Master contains all components for PCR, except DNA templates and primers. The mixture is suitable for amplification of most of the DNA templates.
    In addition to 5´ to 3´ DNA polymerase activity, Pfu DNA Polymerase also possesses 3´ to 5´ exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

    • Overview
      • Including loading dye for easy gel electrophoresis
      • Thermal stability : Half-life of 40 min at 95°C
      • A-tail formation at 3’ ends of amplified duplex DNA
      • Ready-to-use solution
      • Easy reaction setup
      • High fidelity PCR amplification
      • Room-temperature reaction set-up
      • Contains loading dye for visual confirmation and quick electrophoresis
      • Minimal optimization requirements
    • Ordering Information
      Cat No Size Remark
      W1451 1 ml 100 rxn
      W1451-5 5 x 1 ml 500 rxn
    • Technical Data
    • Applications
      • High fidelity PCR for cloning
      • Amplification of long DNA fragments ( >5 ~ 15 kb )
      • DNA amplification to generate blunt end products
      • Site-directed mutagenesis
      • DNA sequencing
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice