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  • PCR > FDmix (Dried type)

    WizPure™ HS-PCR FDmix

    WizPure™ HS-PCR FDmix combines all the reagents necessary for successful antibody-based hot-start PCR in a convenient individually aliquoted and lyophilized in single-tube. WizPure™ HS-PCR FDmix is an optimized, economical, highly efficient, and ready-to-use hot-start PCR premix that can amplify templates of up to 5 kb. The PCR mixture of hot-start Taq DNA Polymerase, PCR buffer, MgCl2, and dNTPs. This HS-PCR FDmix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates. Tested for the absence of endonucleases & exonucleases and is also tested for amplification of single gene copy.
    WizPure™ HS-PCR FDmix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR, and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.

    • Overview
      • Including loading dye for easy gel electrophoresis
      • A-tail formation at 3’ ends of amplified duplex DNA
      • Ready-to-use dried type
      • Easy reaction setup (pre-aliquot in 8-strip tube)
      • High PCR specificity and high sensitivity PCR amplification
      • Antibody-mediated hot-start PCR
      • Room-temperature reaction set-up
      • Contains loading dye for visual confirmation and quick electrophoresis
      • Minimal optimization requirements
      • Eliminating extra pipetting steps and multiple reagents reduces contamination risk and provide better reproducibility
    • Ordering Information
      Cat No. Size Remark
      W1611 96 T 8 strip x 12 ea
      W1611-5 480 T 8 strip x 60 ea
    • Technical Data
    • Applications
      • High through-put PCR
      • Routine diagnostic PCR requiring high reproducibility
      • Hot-start PCR
      • Primer extension
      • Multiplex PCR
      • High specific amplification of DNA fragments shorter than 3kb
    • Storage & Shipping
      Shipped at room temperature
      Store at -20ºC, upon receipt