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  • Enzyme > DNA Polymerase

    WizPure™ HS-Taq DNA Polymerase

    WizPure™ HS-Taq DNA Polymerase is an antibody-mediated hot-start Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR, and multiplex PCR. The enzyme is a highly processive 5’→3’ DNA polymerase that lacks 3’→5’ exonuclease activity and lacks a 3’→5’ proofreading function.

    • Overview
      • Error rate : 2.4 X 10-5
      • Thermal stability : Half-life of 40 min at 95°C
      • A-tail formation at 3’ ends of amplified duplex DNA
      • Recombinant proteins expressed in E. coli
      • Free of detectable non-specific nuclease 
    • Ordering Information
        Cat No.       Size       Note
      W1311E 500 U without dNTPs
      W1311E-5 2,500 U without dNTPs
      W1311 500 U with dNTPs
      W1311-5 2,500 U with dNTPs
      1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
    • Features
      500 U: 
      • 1 x 100 µl, HS-Taq DNA Polymerase (5U/µl) 
      • 2 x 1 ml, 10X PCR Buffer
      • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

      2,500 U:
      • 1 x 500 µl, HS-Taq DNA Polymerase (5U/µl) 
      • 10 x 1 ml, 10X PCR Buffer 
      • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
    • Technical Data
    • Application
      • Routine PCR
      • Routine diagnostic PCR requiring high reproducibility
      • DNA sequencing template preparation
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice