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  • Enzyme > Reverse Transcriptase

    WizScript™ III Reverse Transcriptase

    - Thermostable RTase
       ; Enables cDNA synthesis up to 60°C, minimizing RNA secondary structures.
    - High fidelity.
    - Versatile primer compatibility.

    WizScript™ Reverse Transcriptase is a genetically modified M-MLV RTase that lacks RNase H activity, enabling efficient full-length cDNA synthesis from RNA or DNA templates. The absence of RNase H prevents degradation of RNA in cDNA/RNA hybrids, improving downstream PCR performance.

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    • Overview
      • RNase H negative
      • Molecular weight: 71 kDa
      • Reaction temperature: 40°C - 45°C
      • Heat inactivation: 70°C / 10 min.
      • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
      • 200 units/μl
      • High-purity enzyme completely free of E.coli DNA contamination
      • GMP grade
    • Technical Data
    • Application
      • Gene synthesis
      • First-strand synthesis of cDNA
      • RT-PCR
      • Random priming reactions
      • Library construction
      • Probe labeling
      • mRNA 5’-end mapping
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice
    • Ordering Information
      Cat No. Size 가격(원) Remark Note
      W2303 10,000 U 90,000 200U/μl
      W2303-5 50,000 U 360,000 200U/μl