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  • Enzyme > DNA Polymerase

    WizPure™ Bst DNA Polymerase (Large Fragment, exo-)

    WizPure™ Bst DNA Polymerase (Large Fragment, exo-) is a DNA polymerase from Bacillus stearothermophilus that is modified so that it retains its 5’→ 3’ DNA polymerase activity while lacking the intrinsic 5’→ 3’ exonuclease domain. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.
     

    • Overview
      • 5'→3' DNA polymerase activity and strand-displacement activity
      • DNA synthesis can be performed at a constant temperature
      • Suitable for synthesis of DNA strands having high GC content
      • Not affected by inhibition due to the secondary structure of DNA
      • High purity/high-quality control
    • Ordering Information
      Cat No. Size Remark
      W1321 2,000 U
      W1321-5 10,000 U
    • Features
      2,000 U contains: 
      1 x 250 µl, Bst DNA Polymerase (8U/µl)
      2 x 1.5 ml, 10X Bst Reaction Buffer 

      10,000 U contains: 
      1 x 1,250 µl, Bst DNA Polymerase (8U/µl)
      7 x 2 ml, 10X Bst Reaction Buffer
    • Figures
    • Applications
      • DNA sequencing through high GC regions
      • Rapid Sequencing from nanogram amounts of DNA template
      • Loop-mediated isothermal amplification (LAMP)
      • Whole genome amplification (WGA)
      • Ramification amplification (RAM)
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice