WizPrep™ Gel/PCR Purification Mini Kit was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. The unique dual-purpose application and high yield DNA/ minicolumn make this kit an exceptional value.
The method uses a chaotropic salt, guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by the addition of a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.
|Contents||100 prep||300 prep|
|GP Buffer||60 ml||180 ml|
|Wash Buffer||16 ml||50 ml|
|Elution Buffer||10 ml||30 ml|
|Spin Columns||100 ea||300 ea|
|Collection Tubes (2.0 ml)||100 ea||300 ea|