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  • Enzyme > DNA Polymerase

    WizPure™ Taq DNA Polymerase

    WizPure™ Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase. The 94 kDa protein possesses an inherent 5’→3’ nick-translation moiety and lacks a 3’→5’ proofreading function.

     

    • Overview
      • Error rate : 2.4 X 10-5
      • Thermal stability: Half-life of 40 min at 95°C
      • A-tail formation at 3’ ends of amplified duplex DNA
      • Recombinant proteins expressed in E. coli  
      • Free of detectable non-specific nuclease
    • Ordering Information
      Cat No. Size   Note  
      W1301E 500 U without dNTPs
      W1301E-5 2,500 U without dNTPs
      W1301 500 U with dNTPs
      W1301-5 2,500 U with dNTPs
      1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
    • Features
      500 U contains: 
      • 1 x 100 µl, Taq DNA Polymerase (5U/µl) 
      • 2 x 1 ml, 10X PCR Buffer 
      • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

      2,500 U contains:
      • 1 x 500 µl, Taq DNA Polymerase (5U/µl) 
      • 10 x 1 ml, 10X PCR Buffer 
      • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
    • Technical Data
    • Application
      • Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)
      • Primer extension
      • Colony PCR
      • Multiplex PCR
      • Labeling of DNA fragments with radioactive-isotopes Nucleotide sequencing
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice