Real-Time PCR Kit for Multidrug-Resistant Tuberculosis Detection
The EuDx™ MDR-TB Detection Kit is a ready-to-use real-time PCR assay for the qualitative detection of Mycobacterium tuberculosis and key drug resistance markers associated with multidrug-resistant TB (MDR-TB). The kit delivers high sensitivity and specificity with a simplified workflow, enabling reliable identification of drug resistance in clinical specimens.
Simultaneous detection of Mycobacterium tuberculosis and Multi-drug-resistant Mycobacterium tuberculosis (MDR-TB), including resistance to rifampicin (RIF) and isoniazid (INH)
Ready-to-use mix with minimal preparation
High sensitivity and specificity
Internal control is included for quality assurance
Uracil-DNA glycosylase (UDG) system is incorporated to prevent carryover contamination
Hot-start PCR technology minimizes non-specific amplification and enhances specificity.
Long shelf life: 15 months.
Protocol
Technical Data
Sensitivity The assay demonstrates positive detection rates of over 95% at 100 copies for all 12 target mutations. Furthermore, key mutations such as rpoB S531L, katG S315T, and inhA-15C are reliably detected at as low as 50 copies.
Specificity No cross-reactivity was observed with genomic DNAs from 13 non-tuberculous mycobacterial (NTM) species and 14 other pathogenic microorganisms. Additionally, common PCR inhibitors, including human genomic DNA and certain antibiotics, did not interfere with assay performance.
