자산 1
ENG KOR
ENG KOR
  • Enzyme > Reverse Transcriptase

    WizPure™ MMLV RTase (RNase H+)

    WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.
    This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

    • Overview
      • 5' to 3' exonuclease: No
      • 3' to 5' exonuclease: No
      • 3’ – A Overhang: No
      • Fragment Size: Up to 10 kb
      • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
      • 200 units/μl
    • Ordering Information
      Cat No. Size Remark
      W2301 10,000 U 200U/μl
      W2301-5 50,000 U 200U/μl
      1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
    • Features
      10,000 U contains: 
      1 x 50 μl, MMLV RTase (RNase H+)
      1 x 500 μl, 5X Reaction Buffer  

      50,000 U contains: 
      1 x 250 μl, MMLV RTase (RNase H+)
      1 x 2,500 μl, 5X Reaction Buffer 
    • Technical Data
    • Application
      • Gene synthesis
      • First-strand synthesis of cDNA
      • RT-PCR
      • Random priming reactions
      • Library construction
      • Probe labeling
      • mRNA 5’-end mapping
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice