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  • Enzyme > Reverse Transcriptase

    WizScript™ III Reverse Transcriptase

    WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase that exhibits RNA or DNA-dependent DNA polymerase but lacks ribonuclease H activity.
    This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.

    • Overview
      • RNase H negative
      • Molecular weight : 71 kDa
      • Reaction temperature : 37°C ~ 42°C
      • Heat inactivation : 70°C / 10 min.
      • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
      • 200 units/μl
    • Ordering Information
      Cat No. Size Remark
      W2303 10,000 U 200U/μl
      W2303-5 50,000 U 200U/μl
    • Features
      10,000 U contains: 
      1 x 50 μl, WizScript™ III Reverse Transcriptase
      1 x 500 μl, 5X Reaction Buffer [250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, 50 mM dithiothreitol, pH 8.3 (25°C
      )]

      50,000 U contains:
      5 x 50 μl, WizScript™ III Reverse Transcriptase
      5 x 500 μl, 5X Reaction Buffer
    • Technical Data
    • Application
      • Gene synthesis
      • First-strand synthesis of cDNA
      • RT-PCR
      • Random priming reactions
      • Library construction
      • Probe labeling
      • mRNA 5’-end mapping
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice