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  • Enzyme > DNA Polymerase

    WizPure™ FX-Taq DNA Polymerase

    WizPure™ FX-Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase and 3’→5’ proofreading function. The FX-Taq DNA polymerase optimized for PCR amplification of genomic DNA templates up to 20 kb and lambda DNA up to 30 kb. With its enhanced processivity, yield, speed, and excellent 3’→5’ exonuclease and 3′→5′ proofreading activity, this enzyme are able to consistently deliver accurate and reliable amplification of long templates.
    This product is the ideal choice for long DNA templates unable to be amplified in conventional PCR and is highly suitable for multiple downstream applications including complex cloning and genotyping experiments. The PCR product amplified with this mixture has one A added at 3’-end, so the product can be directly used for TA cloning.

    • Overview
      • Error rate : 1.3 x 10-6
      • Thermal stability : Half-life of 4 min at 95°C
      • A-tail formation at 3' ends of amplified duplex DNA
      • Recombinant proteins expressed in E. coli
      • Free of detectable non-specific nuclease
    • Ordering Information
      Cat No. Size Remark Contents
      W1341ES 250 U without dNTPs   - 1 x 100µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 1 x 1ml, 10X PCR Buffer
        - 1 x 0.5ml, 25 mM MgCl2 
      W1341E 500 U without dNTPs   - 1 x 200µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 2 x 1ml, 10X PCR Buffer 
        - 1 x 1ml, 25 mM MgCl2 
      W1341E-5 2,500 U without dNTPs   - 1 x 1,000µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 10 x 1ml, 10X PCR Buffer 
        - 5 x 1ml, 25 mM MgCl2 
      W1341S 250 U with dNTPs   - 1 x 100µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 1 x 1ml, 10X PCR Buffer
        - 1 x 0.5ml, 25 mM MgCl2 
        - 1 x 0.5ml, dNTP mix (2.5 mM each)
      W1341 500 U with dNTPs   - 1 x 200µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 2 x 1ml, 10X PCR Buffer 
        - 1 x 1ml, 25 mM MgCl2 
        - 1 x 1ml, dNTP mix (2.5 mM each)
      W1341-5 2,500 U with dNTPs   - 1 x 1,000µl, FX-Taq DNA Polymerase (2.5U/µl) 
        - 10 x 1ml, 10X PCR Buffer 
        - 5 x 1ml, 25 mM MgCl2 
        - 5 x 1ml, dNTP mix (2.5 mM each)
    • Technical Data
    • Applications
      • Long range PCR
      • High-fidelity PCR
      • Primer-extension reaction
      • Genotyping
      • Library construction
      • High GC amplification
      • Next-generation DNA sequencing
      • TA cloning
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice