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  • Real-Time PCR > Real-Time PCR

    WizPure™ qPCR Master-UDG (PROBE)

    WizPure™ qPCR Master-UDG (PROBE) is a ready-to-use 2X reagent ideal for most quantitative real-time PCR applications. The master mix is recommended for use with Labeled Fluorescent Probes, e.g. for 5’-Nuclease Assays or Hybridization probes. The qPCR Master-UDG (PROBE) contains an antibody-mediated hot-start Taq DNA Polymerase, MgCl2, dATP, dCTP, dGTP, dUTP, Uracil DNA Glycosylase (UDG), enhancer, and stabilizer. 
    UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single or double-stranded DNA, preventing dU-containing DNA from serving as a template in future PCRs.

    • Overview
      • Ready-to-use solution
      • Easy reaction setup
      • Minimal optimization requirements
      • Antibody-mediated hot-start PCR
      • For universal Real-time PCR master mix
      • Suitable for most Real-time PCR machine
      • Applied unique enhancing & stabilizing technology
    • Ordering Information
      Cat No. Size Remark
      W1702 1 ml 100 rxn
      W1702-5 5 x 1 ml 500 rxn
      W1702R 1 ml 100 rxn with ROX dye
      W1702R-4 4 x 1 ml 400 rxn with ROX dye
    • Features
      W1702:
      1 ml, qPCR Master-UDG (PROBE)

      W1702-5:
      5 x 1 ml, qPCR Master-UDG (PROBE)

      W1702R:
      1 ml, qPCR Master-UDG (PROBE)
      50 μl, ROX Dye (50X)

      W1702R-4:
      4 x 1 ml, qPCR Master-UDG (PROBE)  
      200 μl, ROX Dye (50X)
    • Technical Data
    • Applications
      • High through-put Real-time PCR
      • Gene expression profiling
      • Gene knockdown verification
      • Array validation
      • Bacterial & Viral detection
      • Diagnostic purpose
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice