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  • Real-Time PCR > Real-Time PCR

    WizPure™ qPCR Master (EVA)

    WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: WizPure™ HS-Taq DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen dye. The user simply needs to add water, template, and primers. Hot start DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 
    The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.

    • Overview
      • Ready-to-use solution
      • Easy reaction setup
      • Minimal optimization requirements
      • Antibody-mediated hot-start PCR
      • For universal Real-time PCR master mix
      • Suitable for most Real-time PCR machine
      • Applied unique enhancing & stabilizing technology
    • Ordering Information
      Cat No. Size Remark
      W1721 1 ml 100 rxn
      W1721-5 5 x 1 ml 500 rxn
      W1721R 1 ml 100 rxn with ROX dye
      W1721R-4 4 x 1 ml 400 rxn with ROX dye
    • Features
      W1721:
      1 ml, qPCR Master (EVA)

      W1721-5:
      5 x 1 ml, qPCR Master (EVA)

      W1721R:
      1 ml, qPCR Master (EVA)
      50 μl, ROX Dye (50X)

      W1721R-4:
      4 x 1 ml, qPCR Master (EVA)
      200 μl, ROX Dye (50X)
    • Technical Data
    • Applications
      • Real-time PCR
      • Detection and quantification of DNA and cDNA targets
      • Gene expression profiling & knockdown verification
      • Microbial detection
      • Viral load determination
      • Array validation
      • SNP genotyping
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice