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  • Enzyme > DNA Polymerase

    WizPure™ Pfu DNA Polymerase

    WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction.
    Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

    • Overview
      • Error rate : 1.3 x 10-6
      • Time to 1kb (72°C) : 60-120
      • Thermal stability : Half-life of 4 hrs at 95°C
      • Blunt end products
      • Recombinant proteins expressed in E. coli
      • Free of detectable non-specific nuclease 
    • Ordering Information
      Cat No. Size Remark
      W1330E 250 U without dNTPs
      W1331E 500 U without dNTPs
      W1331E-5 2,500 U without dNTPs
      W1330 250 U with dNTPs
      W1331 500 U with dNTPs
      W1331-5 2,500 U with dNTPs
      Unit Definition 1 unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
    • Features
      250 U contains: 
      • 1 x 100 µl, Pfu Polymerase (2.5U/µl) 
      • 1 x 1 ml, 10X PCR Buffer 
      • [ 1 x 0.5 ml, dNTP mix (2.5 mM each) ]

      500 U contains: 
      • 1 x 200 µl, Pfu Polymerase (2.5U/µl) 
      • 2 x 1 ml, 10X PCR Buffer 
      • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

      2,500 U contains: 
      • 1 x 1,000 µl, Pfu Polymerase (2.5U/µl) 
      • 8 x 1 ml, 10X PCR Buffer 
      • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
    • Technical Data
    • Applications
      • High fidelity PCR for cloning
      • Amplification of long DNA fragments (>5-15 kb)
      • DNA amplification to generate blunt end products
      • Site-directed mutagenesis
      • DNA sequencing
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice