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  • Real-Time PCR > Real-Time PCR

    WizPure™ qPCR Master (SYBR)

    WizPure™ qPCR Master (SYBR) with ROX Dye is an optimized ready-to-use solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody-mediated hot-start Taq DNA Polymerase, ultra-pure dNTPs, MgCl2, SYBR Green I, enhancer, and stabilizer. Hot start DNA Polymerase is activated by a 5-minute incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
    WizPure™ qPCR Master (SYBR) Lo-ROX kit and WizPure™ qPCR Master (SYBR) Hi-ROX kit are optimized ready-to-use 2X master mix solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2, SYBR Green I, enhancer, stabilizer, and ROX reference dye ensures that the kits deliver fast, highly-specific and ultra-sensitive real-time PCR.
     

    • Overview
      • Ready-to-use solution
      • Easy reaction setup
      • Minimal optimization requirements
      • Antibody-mediated hot-start PCR
      • For universal Real-time PCR master mix
      • Suitable for most Real-time PCR machine
      • Applied unique enhancing & stabilizing technology
    • Ordering Information
      Cat No. Size Remark
      W1711 1 ml 100 rxn
      W1711-5 5 x 1 ml 500 rxn
      W1711R 1 ml 100 rxn with ROX dye
      W1711R-4 4 x 1 ml 400 rxn with ROX dye
      W1711RL 1 ml Low ROX dye
      W1711RL-5 5 x 1 ml Low ROX dye
      W1711RH 1 ml High ROX dye
      W1711RH-5 5 x 1 ml High ROX dye
    • Features
      W1711:
      1 ml, qPCR Master (SYBR)

      W1711-5:
      5 x 1 ml, qPCR Master (SYBR)

      W1711R:
      1 ml, qPCR Master (SYBR)
      50 μl, ROX Dye (50X)

      W1711R-4:
      4 x 1 ml, qPCR Master (SYBR)  
      200 μl, ROX Dye (50X)

      W1711RL:
      1 ml, qPCR Master (SYBR) Lo-ROX

      W1711RL-5:
      5 x 1 ml, qPCR Master (SYBR) Lo-ROX

      W1711RH:
      1 ml, qPCR Master (SYBR) Hi-ROX

      W1711RH-5:
      5 x 1 ml, qPCR Master (SYBR) Hi-ROX 
       
    • Technical Data
    • Applications
      • High through-put Real-time PCR
      • Detection and quantification of DNA and cDNA targets
      • Gene expression profiling
      • Gene knockdown verification
      • Microbial detection
      • Viral load determination
      • Array validation
      • SNP genotyping
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice