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  • Real-Time PCR > Real-Time PCR

    WizPure™ qPCR Master (Super Green)

    WizPure™ qPCR Master (Super Green) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating Super Green dye. It comprises all the components necessary to perform qPCR: antibody-mediated hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2, Super Green, enhancer, and stabilizer. The user simply needs to add water, template, and primers. Hot start DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 
    The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
     

    • Overview
      • Ready-to-use solution
      • Easy reaction setup
      • Minimal optimization requirements
      • Antibody-mediated hot-start PCR
      • For universal Real-time PCR master mix
      • Suitable for most Real-time PCR machine
      • Applied unique enhancing & stabilizing technology
    • Ordering Information
      Cat No. Size Remark
      W1731 1 ml
      W1731-5 5 x 1 ml
    • Technical Data
    • Applications
      • High through-put Real-time PCR
      • Detection and quantification of DNA and cDNA targets
      • Gene expression profiling
      • Microbial detection
      • Viral load determination
      • Array validation
      • SNP genotyping
    • Storage & Shipping
      Store at -20ºC in a frost-free freezer
      Shipped on ice packs and/or dry ice